Translational_Unit

Part:BBa_K1500004

Designed by: Kevin Yang   Group: iGEM14_UChicago   (2014-10-17)


IPTG-GFP MutD

The MutD gene cloned into the IPTG-GFP plasmid for characterization. It is unmutated and still requires the MutD51 (L73W) mutation to become functional in its mutagenic dominant negative form. MutD (dnaQ) gene product controls the editing capacity of pol III (a proofreading DNA exonuclease) and mutD5 mutation leads to a reduction in mismatch repair.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1147
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1019
    Illegal AgeI site found at 1508
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 870


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Categories
Parameters
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